For the first time, archaeologists have analyzed the genetic material of Homo naledi, a mysterious 300,000-year-old relative of modern humans discovered deep in a South African cave system. What they found is unique in human evolution studies: Every skeleton known from the species is female.

"I think it is fair to say that they surprised us," Lee Berger, a National Geographic explorer-in-residence, told Live Science in an email, but H. naledi "has always been an enigmatic discovery."

Since 2013, Berger has headed the Rising Star project, which discovered nearly two dozen skeletons of small-brained, two-legged creatures, which the research team named H. naledi, within a cave system in South Africa's Cradle of Humankind.

Research over the past decade has revealed that H. naledi was unusual for having a small brain and upper body, similar to earlier australopithecines like Lucy, but a face, hands and lower limbs that were more human-like. In 2023, the Rising Star team suggested H. naledi may have used fire in the cave, and in 2025, they advanced the controversial claim that H. naledi buried their dead — a complex behavior unexpected for a human relative with such a small brain.

But a new study of H. naledi teeth published Wednesday (June 24) in the journal Cell may bolster the team's interpretation of the Rising Star cave as a burial site.

An international team of experts studied 20 teeth from H. naledi skeletons using proteomic analysis, a minimally destructive technique that sequences genetic material from ancient proteins. Proteomics is a burgeoning field, especially because these proteins can last longer than DNA. The team focused on amelogenin genes (AMEL), which code for proteins in dental enamel and vary by sex. While the gene variant called AMELX is found in both males and females, another one, AMELY, is found only in biological males.

In analyzing the H. naledi teeth, the team found no AMELY genes but plenty of AMELX ones, suggesting that all of the skeletons were from females. These included the nearly complete skeleton of Neo and DH1, the main representative of the species, both originally assumed to be male.

The result is surprising because there are no known ancient human cemeteries or collections of nonhuman primate skeletons that contain only females.

"The most likely reason for these robust results are, in my opinion, cultural selection after death for burial by sex and perhaps gender," Berger said. "There are many past human societies with sex-specific burial practices," study co-author John Hawks, a paleoanthropologist at the University of Wisconsin-Madison, said in a statement, but the H. naledi skeletons "are older than any known Neanderthal or modern human burial site, and it's remarkable to see that they may all be female."

"An already weird hominin"

The discovery that everything we know about H. naledi comes from female skeletons has surprised paleoanthropologists.

"The bottom line is this is a weird result from an already weird hominin," Elizabeth Sawchuk, curator of human evolution at the Cleveland Museum of Natural History, who was not involved in the study, told Live Science in an email. "The key thing to remember is that failure to detect evidence of AMELY does not mean there are no males in the sample — it just means that none were detected."

One possible reason for the lack of this gene in H. naledi skeletons is an AMELY gene deletion that is known to occur very rarely in some modern-human populations and that has been found in one Neanderthal male. If the AMELY gene doesn't exist in this H. naledi group, then the protein profiles of males would look identical to the profiles of females.

However, "it's very unlikely that this would be the case among even half of the 20 individuals we studied or for an entire population," study co-author Enrico Cappellini, a paleoproteomics professor at the University of Copenhagen in Denmark, said in the statement. "Either scenario, namely the absence of H. naledi males in the Rising Star cave system or a systematic deletion of their AMELY gene, is fascinating and would have deep implications for a better understanding of the biology and evolution of this species."

Studies of H. naledi, a species known from a single site, "continue to yield more questions than answers," Sawchuk said. "As the authors point out, this is a surprising result that requires more investigation."

Other hominins in South Africa

A second surprising result in the proteomic analysis was that H. naledi shares a gene variant with Paranthropus robustus, a human relative with a massive face and teeth that lived in South Africa around 1 million to 2 million years ago.

Proteomic analysis of four P. robustus skeletons in 2025 proved that limited genetic material could be recovered from ancient human relatives in Africa. The new study has revealed that some members of this species and H. naledi shared a gene variant related to collagen production, which is different from the genes found in modern humans, Neanderthals and Denisovans.

While H. naledi and P. robustus inhabited the same general geographic area, it is unclear if they lived there at the same time and overlapped or if they may have had an ancestor-descendant relationship.

"It is early days for sampling fossil hominins with ancient proteins, and until we build a better, bigger sample, we just don't know" what the shared genetic variant means, Berger said.

Building a larger database of ancient proteins from other human relatives that evolved in Africa, such as Australopithecus africanus and Homo erectus, may clarify where H. naledi fits into the picture of human evolution.

"Key data are missing from H. erectus and A. africanus that would help put this evidence into context," Sawchuk said. "For now, this is another curious finding that bears further investigation."

What does sex change?

In 2015, Berger and colleagues named the new hominin H. naledi and described what they presumed to be male and female variants of the species based on the skeletons' sizes. In many groups of human relatives and in modern humans, males are physically larger than females, on average. This assumption led the researchers to classify the presumed male individual DH1, discovered in the Dinaledi chamber of the cave, as the main representative of the new species.

But a 2024 study was the first to question the assumption that the H. naledi skeletons came from two sexes. In that study, researchers found variation in the teeth of H. naledi that was "so low that the possibility that one sex is represented by few or no individuals in the sample cannot be excluded," they wrote.

"Our study helps resolve the long-standing mystery of why Homo naledi lacked significant variation," study first author Palesa Madupe, a researcher at the Max Planck Institute for Evolutionary Anthropology in Germany, said in the statement. "It's probably because they could have all belonged to one sex."

If the proteomic sex analysis is correct and H. naledi does not have AMELY deletion issues, it means everything we know about the species comes from females. But this doesn't mean interpretations of the species are wrong.

"The only thing that has changed is that we have never seen a male!" Berger said. "When and if we do, we will have to extend the description to include male sex characters and the likely extension of certain aspects of variation."

The researchers hope their study paves the way for more proteomic analyses of human relatives in the future.

The new analysis proves that protein analysis of fossils from the Pleistocene (2.58 million years ago to 11,700 years ago) can be done in a minimally destructive way, Madupe said. "This means potentially opening the door to a whole new way of sustainably investigating the differences between sexes in groups of extinct hominins and other animals without causing visible damage to these priceless fossils."

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Oil lamps have been simple and popular light sources for more than three millennia. But during the Roman and Byzantine empires, many oil lamps were highly decorated works of art. This hanging lamp in the collection of the Metropolitan Museum of Art was made nearly 1,600 years ago in the shape of a human right foot wearing a sandal, likely as an early Christian symbol.

The bronze oil lamp is significantly smaller than a life-size foot, measuring just 3.25 inches (8.3 centimeters) long. Still attached to the lamp is a chain with a hook for hanging, which is over 17 inches (43.5 cm) long.

The right big toe rests against the spout of the lamp, which would have held the wick. A sandal covers the foot, its leather or cord thongs tied at the ankle. The sole of the sandal is decorated with a swastika, which in Byzantine times was known as a gammadion cross and represented good fortune.

At the back of the lamp, there is an opening at the ankle where the lamp could be filled with oil. The flat cover for the opening is topped with a cross, identifying the lamp as a Christian artifact, Vera Ostoia, a curator of medieval art at The Met, wrote in a 1969 study of objects from the The Met Cloisters.

The foot shape may have been a protective image that symbolized good health and healing, according to The Met, and it may have doubled as a symbol of Christian pilgrimage. But the symbolism may have run even deeper.

In early Christian times, oil lamps and the light they produced were metaphors for enlightenment and immortality rather than just functional, Ostoia wrote. This foot lamp may have been connected to Psalm 119:105, which reads: "Thy word is a lamp unto my feet, and a light unto my path," meaning that people should follow the word of God as their path in life.

For more stunning archaeological discoveries, check out our Astonishing Artifacts archives.

Archaeologists have discovered an iron cannonball fired by Texans at the Battle of the Alamo in 1836. This is the second intact cannonball they have discovered from the famous conflict. The first one, a bronze cannonball, was found earlier this year and was likely fired by the Mexican army.

"It's a huge deal," Tiffany Lindley, director of archaeology at the Alamo, said in a video statement from Alamo Trust. "We all thought, 'There's no way we can top it,'" she said, referring to the bronze cannonball discovered March 5, just a day before the 190th anniversary of the conflict that killed Davy Crockett, "and then we found another one."

Both cannonballs were recovered from the northeast corner of the church at the Alamo, which was originally built in 1718 as a Spanish mission and fortress in what is now San Antonio. But the Alamo is better known as the location of an 1836 battle during the Texas Revolution, in which Anglo-American settlers and Hispanic Texans known as Tejanos seceded from the Republic of Mexico.

In a 13-day siege culminating in a deadly assault on March 6, 1836, Mexican troops, commanded by Gen. Antonio López de Santa Anna, surrounded the Alamo and killed the 180 Texan rebels defending the structure, including Davy Crockett. The cry "Remember the Alamo!" was used in later skirmishes in the Texas Revolution as soldiers fought Mexican troops for independence.

The newly excavated iron cannonball was found June 2, according to a statement from Alamo Trust.

Of the two cannonballs, "the newest one is slightly larger" and was likely fired from a cannon that shot 6-pound [2.7 kilogram] cannonballs, Kolby Lanham, a senior researcher and historian at the Alamo, said in the statement. "The likelihood is that the bronze one belonged to the Mexican Army and the iron one belonged to the Texans."

Over the years, archaeologists have found numerous pieces of ammunition and shrapnel at the Alamo, Lindley said, but these cannonballs are the first examples of solid shots — spherical projectiles fired from a gun or cannon — they have ever found.

"It's just a huge kind of once-in-a-lifetime deal," Lanham said. "But obviously, it's happened twice."

The Mexican and Texan cannonballs were found near each other, so they are likely the historic remains of the two sides shooting at each other in the fateful siege nearly two centuries ago.

"Whenever they were dropped the very first time — possibly in 1836, probably — they haven't been touched since, and that's what makes them extra special," Lindley said. "I don't think you can undersell it."

The Alamo remains an active archaeological site, where experts have discovered artifacts that span its centuries of history, from Indigenous stone tools that predate the Spanish mission through the late 19th century, when the structure was converted into a general store and warehouse.

Can you identify these historical objects of war? Test your smarts with our weapons of the world quiz!

Archaeologists in Germany have unearthed an unusual burial of an injured man who was thrown into an abandoned kiln nearly 5,000 years ago. Because archaeologists don't typically find artifacts or bones in kiln pits, they suspect the man may have been a human sacrifice.

While workers were constructing a power line near Gerstewitz, a village in central Germany, archaeologists found a Corded Ware culture grave, according to a June 15 statement from the State Office for Heritage Management and Archaeology (LDA) Saxony-Anhalt.

The Corded Ware culture, named after the distinctive cord-like impressions found in their pottery, spread across Northern Europe between the Late Neolithic and the Bronze Age, around 2900 to 2050 B.C. Graves of Corded Ware people tend to follow a strict pattern: Men were buried on their right side, women were interred on their left, and everyone was buried in a crouched position facing south. But sometimes, graves deviate from this pattern.

The newly excavated grave at Gerstewitz revealed the skeleton of a man who was about 25 years old when he died. He was buried in a crouched position facing south, but rather than being placed in a grave or under a small mound, he was found in a kiln pit. This pit, essentially a hole that had been dug into the ground, held ancient evidence that people had used it to burn material, such as wood or food, or to fire clay. Additionally, the man's skull showed signs of injury.

Experts have suggested three possible explanations for the unusual burial. The skull injury may have resulted from violence, meaning someone murdered the man. Alternatively, he could have perished in a Copper Age battle lost to history. But a third possibility is that he was placed in a kiln pit as a human sacrifice.

Oliver Dietrich, an archaeologist at LDA Saxony-Anhalt, wrote in the statement that, in rare cases, Corded Ware kiln pits contain cow or dog bones thought to be sacrificial offerings to unknown deities. While it's possible this man was a human sacrifice, Dietrich noted that future laboratory analysis should provide more information about the grave and the person buried there.

This is not the first time a sacrificial pit has been found in Gerstewitz. Last year, archaeologists with LDA Saxony-Anhalt discovered 12 pits ringed by a moat-like ditch. The pits were full of dog bones, human skulls and complete ceramic pots and had been sealed with the rubble of burnt houses, according to a 2025 statement about that discovery. An additional human skeleton was found buried in a converted oven pit. These finds, however, were attributed to the Salzmünde Culture (3400 to 3050 B.C.), which predated the Corded Ware people.

Other discoveries near Gerstewitz have revealed that the area was settled and occupied for over 6,000 years. The excavation there will last until at least 2027, when the power line project is expected to be completed.

What do you know about the Paleolithic, Mesolithic and Neolithic eras? Find out with our Stone Age quiz!

A clandestine excavation on the outskirts of Rome has brought to light a "splendid villa" that may have belonged to a member of the Antonine imperial family, according to archaeologists with the Special Superintendency of Rome. The remains of the first-century villa, which was previously unknown to archaeologists, include frescoed walls and mosaic floors that showcase the elite social status of the owners.

The villa was discovered at Castel di Guido, a village about 12 miles (19 kilometers) west of the ancient city walls of Rome. In ancient times, the area was known as Lorium, and it was frequented by prestigious Roman families, including the Roman emperors Hadrian, Antoninus Pius and Marcus Aurelius.

In February, the local police were alerted to illegal excavations at Castel di Guido on government-owned land, according to a June 15 translated statement from the Italian Ministry of Culture's Special Superintendency of Rome. The unauthorized excavations had been undertaken with a backhoe, which made deep cuts into the villa and left huge piles of dirt.

Superintendency archaeologists led by Alessia Contino carried out emergency excavations to document and stabilize the architecture at the site.

"Thanks to the local report and prompt intervention, it was possible to identify part of a large, previously unknown Imperial-period villa and uncover a splendid array of decorations, as well as the discovery of a statue in fine white marble," Contino said in the statement.

The excavation revealed the atrium at the entrance of the villa, two rooms decorated with frescoes and mosaics, and several other spaces related to the agricultural activities of the large, rural house. The villa was likely built in the early first century and abandoned in the third century.

The atrium's central impluvium — a marble basin in the center of many ancient Roman houses that collected rainwater from an opening in the roof — was surrounded by a floor decorated with bands of black-and-white botanical and geometric mosaics. The rear wall of the room, about 5 feet (1.5 meters) of which was still preserved, was painted red as part of a fresco that featured multicolor panels with human figures and botanical elements.

Surrounding the large room were four smaller ones, three of which still have preserved mosaic floors. One room includes nine black-and-white geometric panels, another includes black octagons on a white background, and the third has black rectangles.

Within the villa's central basin, archaeologists found a broken statue, about 31 inches (80 centimeters) tall, depicting a bearded man wearing a short tunic and carrying a basket with birds and fruit. The statue may represent Silvanus, the Roman deity of woods and other uncultivated lands, who watched over domesticated flocks of animals. Silvanus was also a companion of Bacchus (known in Greek as Dionysus), the god of wine and ecstasy.

The scale of the villa and the quality of the mosaics and frescoes suggest that the villa's owners were Roman aristocrats and may have even been members of the imperial family, according to the statement. In the Roman Empire, elite families like the Aurelii and the Arrii — the paternal and maternal families of Antoninus Pius, who reigned from A.D. 138 to 161 — lived in Lorium. Ancient sources suggest that Antoninus Pius spent his childhood in Lorium, built his imperial palace there, and died there at age 74.

Lorium was also frequented by Emperor Hadrian, who adopted Antoninus Pius to make him his heir, and by Marcus Aurelius, who was Antoninus Pius' nephew, adopted son and son-in-law. Because of Lorium's strong connection to the Antonine imperial dynasty, numerous villas and ancient estates have previously been discovered in the region.

But the newly uncovered villa "is another important piece of the puzzle," Contino said, "opening up new perspectives for understanding and preserving our history-rich territory."

Further work on the villa and its artifacts will help archaeologists date the building more accurately and work out whether it was abandoned when the imperial household was relocated out of Lorium, according to the statement.

Although work on the villa has not been completed, the public has a chance to visit the newly excavated villa and its mosaics on June 20 through a free archaeological hike and guided tour organized by the Special Superintendency of Rome.

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