For decades, the painted tombs of Tarquinia, Italy, have been viewed as artwork depicting scenes of music, feasting, processions, and the afterlife that date back to the first millennium BCE.
A recent study took a different approach to interpreting these paintings by examining how the physical characteristics of these tombs might have affected the senses of the ancient people who visited them. Archaeologists Maurizio Forte of Duke University and Jacqueline Ortoleva of the University of Oxford used AI-assisted visual analysis, 3D architectural modeling, and acoustic surveys to reconstruct the physical experience of entering two tombs over 2,400 years old.
The researchers propose that these tombs not only served as burial sites but also as spaces that could shape visitors’ sensory experiences. Their findings, published in the Journal of Archaeological Method and Theory, indicate that the two chambers could have produced contrasting sensory effects.
Forte and Ortoleva prepared detailed three-dimensional models of both tombs and conducted acoustic surveys in order to gain an understanding of the sensory experience. To do this, they played recorded handclaps at different locations within each chamber and measured how the sound decayed and propagated through the space. The results indicated that low-frequency sounds under 1,000 Hz were particularly important in this environment. Forte and Ortoleva stated that people not only hear these sounds but also feel them in the body, where they can cause vibration, disorientation, and possibly alterations in cognitive state.
The team used AI models to identify features that may have attracted attention and to determine where rituals may have taken place. Lighting relied on candlelight, as torches would have produced unsafe amounts of smoke in these confined spaces.
Built between 450 and 400 BCE, the smaller Tomba del Gallo offers a striking contrast to the larger tomb. A steep stairway measuring 3.31 meters long and 2.5 meters wide descends to a burial chamber. The artists painted a banquet scene on one wall of the stairway corridor and musicians playing pipe instruments on the opposite wall.. This stairway leads directly into the main chamber.
In the main chamber, visitors naturally focus on the paintings and on points where the architecture changes. Areas of high curvature where the chamber walls meet function as visual focal points. Visitors may have used these areas for ritual offerings or performed ritual gestures there.
The acoustics in the Tomba del Gallo also suggest this function. The reverberation in the chamber is short and doesn’t allow sound to build up. Instead, it rises and moves away from the space. The researchers’ simulations link the combination of clear sightlines and short reverberation to alpha-band brain activity, often associated with relaxed alertness and focused visual attention. The researchers describe the result as a comparatively calm, focused experience that lets visitors concentrate on the paintings.
The second tomb, Tomba dei Demoni Azzurri, dates to the same period, between 450 and 420 BCE. The burial chamber is much larger, measuring 6.2 by 6.1 meters, almost four times as large as that of Tomba del Gallo.
One image in the tomb depicts a group of demons with a small boat carrying a woman and a smaller man. Etruscan iconography often associated demons with the afterlife.
Compared with the Tomba del Gallo, the acoustics in this chamber are considerably more intense. The reverberation is greater and lasts longer, particularly in the area near the banquet scene, and sound passes easily between the tomb and the outside area. Forte and Ortoleva state that the variety of reverberation times means both low-frequency sounds, such as drumbeats or handclaps, and high-frequency sounds such as voices would have echoed throughout the space, thus producing a far more powerful sonic environment than in the smaller tomb.
“Visual saliency analysis identifies the blue demon on the right wall as an extreme attentional attractor,” Forte and Ortoleva write. “The demon’s chromatic qualities, combined with its aggressive posture and spatial prominence in the center of the burial chamber’s right wall, produce an element of visual surprise. The visual gaze is arrested and attention seems to be forcibly captured. This is paralleled by the acoustics of the tomb.”
The combination of low-frequency vibration, loss of spatial orientation, and increasingly unintelligible speech may have left visitors feeling disembodied and disoriented. The team’s simulations link these effects to beta-band activity and transient gamma bursts, which are associated with heightened alertness and threat detection.
Together, the two tombs suggest that Etruscan mourners moved through carefully arranged environments of candlelight, painted figures, and resonant sound, with each tomb offering a distinct sensory experience. It remains unclear whether the tomb builders intentionally designed these effects or if they simply resulted from the available architecture and materials.
Austin Burgess is a writer and researcher with a background in sales, marketing, and data analytics. He holds an MBA, a Bachelor of Science in Business Administration, and a data analytics certification. His work focuses on breaking scientific developments, with an emphasis on emerging biology, cognitive neuroscience, and archaeological discoveries.
In 2022, when archaeologists uncovered the grave of a young woman buried in 17th-century Pień, Poland, they were confronted with a bizarre scene. Hanging from the big toe of her left foot was an iron padlock. Across her neck lay a large sickle, its blade positioned directly toward her throat.
The strange arrangement suggested that whoever buried her may have feared that, like the vampires of European folklore, she might somehow rise from the grave.
In the four years since “Grave 75” was discovered, scientists have subjected the young woman’s remains, clothing, grave, and the mysterious objects buried with her to an unusually extensive forensic investigation involving ancient DNA, radiocarbon dating, isotope chemistry, X-ray imaging, and microscopic analysis.
Published in the Journal of Archaeological Science: Reports, the findings are beginning to reveal who the mysterious woman was. She was only around 17 to 19 years old when she died, appears to have come from a wealthy or high-status family, and probably spent her childhood in the surrounding region.
Most strikingly, the evidence suggests that local fears surrounding the young woman may have grown after she was already buried.
Researchers believe the padlock was placed on her toe during the first burial. Sometime later, the grave was apparently reopened and the sickle positioned across her neck as an additional safeguard.
The circumstances surrounding the young woman’s death, and why the community apparently feared her enough to take such extraordinary precautions against her trying to return to the land of the living, remain a mystery.
“The burial, uncovered and later disturbed, is distinguished by the co-occurrence of a sickle and a padlock within a single grave, a combination not previously reported in the published archaeological literature,” researchers write. “Ongoing palaeopathological research may shed new light on the cause of death of this young female, which remains a mystery today.”
The grave represents what archaeologists describe as an “anti-demonic” or “anti-vampiric” burial. However, the researchers caution that applying the familiar label of “vampire” to such graves can be misleading.
Although versions of the term vampire can be traced as far back as the Middle Ages, it did not become widespread across Europe until around the 18th century. Earlier Slavic cultures used terms including upiór and strzyga to describe people who, after death, were believed capable of becoming dangerous supernatural beings that could threaten the living.
Researchers have identified several hundred suspected anti-vampire burials dating from the post-medieval period onward across Central and Southeastern Europe.
“Grave 75” was discovered at a cemetery in Pień overlooking the Vistula Valley, where archaeologists have uncovered more than 100 post-medieval graves. Researchers believe the cemetery may have served a Protestant community, and its unusual location away from the village raises the possibility that it was also used to bury people considered social outsiders. Several dozen graves contain unusual features, including deliberately placed stones, coins, sickles, and padlocks.
Sickles and padlocks have both been associated with old European burial practices intended to prevent potentially dangerous dead from returning to harm the living. But finding the two together in one “vampire grave” was unprecedented. No previously published archaeological account had documented a burial containing both a sickle and a padlock.
The woman lay on her back with her legs extended. Around her neck was a 14-inch-long serrated sickle, positioned with its cutting edge toward her throat. Hanging from the big toe of her left foot was a triangular iron padlock. Archaeologists also recovered fragments of an elaborate textile near her skull.
Microscopic analysis showed the material was silk decorated with metal threads made from silver and gilded silver. Such expensive trimmings were generally associated with aristocrats, nobility, and wealthy townspeople. Researchers, therefore, conclude that the teenager likely came from a relatively high social class, making her apparent treatment after death particularly unusual.
Analysis of her skeleton offered few indications about how she died. Researchers estimated that she stood roughly 5 feet 5 inches tall and found no evidence of fatal trauma or skeletal disease that might explain her death. Without any surviving soft tissue, however, determining a definitive cause of death may be impossible.
DNA confirmed the remains belonged to a female and placed her mitochondrial lineage within haplogroup J1c2c1, a lineage found particularly among ancient and modern Scandinavian populations.
Isotope measurements suggest she probably spent her childhood around Pień. One possibility is that she belonged to a family with more distant Scandinavian ancestry, although the researchers caution that genetics and isotopes cannot establish geographic origin with certainty.
More intriguing is evidence that the precautions surrounding her body were not all taken at the same time.
The padlock appears to have been attached when she was originally buried. The sickle may have come later.
Disturbed soil around the vampire grave suggests somebody subsequently reopened it. Because the skeleton remained articulated, researchers believe that, if it were reopened, it probably happened before the body had completely decomposed. At that point, someone may have placed or driven the sickle across her neck as an additional safeguard.
That raises a disturbing possibility that the community already regarded the young woman as potentially dangerous when she died. Yet, for unknown reasons, they became even more frightened of her after her burial.
Historical traditions provide some context. In parts of Poland, a sickle placed across the throat was believed to prevent a strzyga from returning. Birch, one possible wood identified in the sickle’s deteriorated handle, was itself associated with protection against demons and witchcraft.
There may have even been yet another precaution. Extensive green discoloration inside the skull suggests that an object containing copper could have been placed in her mouth. Chemical analysis could not determine what it was, although researchers say it was unlikely to be a coin. Objects inserted into the mouths of suspected revenants are known from other “anti-vampiric” burials.
None of this means the woman was literally considered a “vampire” in the modern Dracula sense. Instead, the evidence points toward a wider fear that certain dead people could return or somehow cause harm.
Why this particular teenager inspired such fear is still a mystery. Researchers suggest her ancestry, young age, an unusual or socially troubling death, disease, or behavior during life could all have contributed.
In periods of epidemic, famine, and other disasters, communities sometimes blamed supposedly dangerous dead people for otherwise inexplicable misfortune. Researchers stress that these explanations remain hypotheses, not proof of what her contemporaries believed.
For now, the only thing that seems certain is that the local community feared this young woman enough to secure her body not only once, but returned to her grave to secure it again.
“Grave 75, as well as other non-normative graves in the cemetery in Pien, demonstrate practices of a protective/anti-demonic nature,” researchers write. “[They] vividly illustrate superstitions, including the tendency to blame the deceased for misfortunes and the fear of the unknown in the 17th-century Polish-Lithuanian Commonwealth.”
The recent study, “A unique, non-normative burial of a young adult female from a 17th-century cemetery in Pień, north-central Poland. A multidisciplinary approach,” appeared in the Journal of Archaeological Science: Reports.
Tim McMillan is a retired law enforcement executive, investigative reporter and co-founder of The Debrief. His writing typically focuses on defense, national security, the Intelligence Community and topics related to psychology. You can follow Tim on Twitter: @LtTimMcMillan. Tim can be reached by email: tim@thedebrief.org or through encrypted email: LtTimMcMillan@protonmail.com
A team of researchers examined 23 teeth from at least 20 individuals from South Africa‘s Rising Star Cave system and found no evidence of the male-specific Amelogenin-Y protein.
A recent study, published in Cell, marks the first ancient protein analysis of Homo naledi and raises the possibility that the Dinaledi Chamber may contain predominantly, or possibly exclusively, female individuals. If confirmed, Homo naledi would become the first known non-human species to practice sex-specific burial.
Since its discovery in 2013, Homo naledi has puzzled paleoanthropologists. This extinct human relative lived between 335,000 and 241,000 years ago and combined ape-like anatomy with distinct human features. So far, Homo naledi remains have only been found in the Rising Star Cave system in South Africa.
For over a decade, scientists noted that the adult fossils from the Dinaledi Chamber were nearly identical, lacking the skeletal variation usually seen between males and females. The reason for this similarity remained unclear.
To investigate further, a team from the University of York, the University of Copenhagen, and additional institutions analyzed the remains at the molecular level. They used a minimally destructive acid-etching method to extract protein fragments, or peptides, from the enamel of the 23 teeth.
“Unlike those found in other remains like bone fragments, proteins in tooth enamel are preserved because dental enamel—the hardest tissue in the human body—shields proteins from environmental contamination even for millions of years. This makes them ideal carriers of genetic information from deep time. Our study helps explain the long-standing mystery of why Homo naledi lacked significant variation; it’s probably because they could have all belonged to one sex,” said Palesa Madupe, who conducted the research during her postdoctoral fellowship at the University of Copenhagen.
The researchers specifically searched for Amelogenin-Y, a protein produced only by the male Y chromosome, but detected none of it in the sampled teeth. To make sure the results were not due to modern contamination, a specialized chemistry team at the University of York independently confirmed that the amino acids were genuinely ancient. The findings remained consistent.
“The lack of male markers within the group is truly fascinating. It is incredibly exciting to gain a window not only into the biology of our ancestors, but also into how they lived. These findings offer rare insights into a culture that has, until now, been difficult to access directly. Advances in ancient protein analysis are opening the door to a far richer and more nuanced understanding of ancient hominins,” said Dr. Marc Dickinson from the university’s Department of Chemistry.
The findings allow for two possible explanations. One is cultural: if the chamber were reserved for females, it could indicate that Homo naledi practiced sex-specific, symbolic mortuary behavior, a trait previously thought to be unique to modern humans.
The second explanation is biological. The researchers note that a small, isolated Homo naledi population could have lost or mutated the Amelogenin-Y gene, leaving males without the protein marker typically used to identify male remains.
Either explanation could reshape what scientists know about Homo naledi. If researchers confirm the site as an all-female burial chamber, it would suggest that symbolic behavior emerged earlier in the human lineage than previously believed. On the other hand, a genetic change across the population would show how isolation can shape a species’ biology in ways that fossils alone cannot reveal. This study shows how ancient protein analysis from teeth can open new windows into human evolution.
Austin Burgess is a writer and researcher with a background in sales, marketing, and data analytics. He holds an MBA, a Bachelor of Science in Business Administration, and a data analytics certification. His work focuses on breaking scientific developments, with an emphasis on emerging biology, cognitive neuroscience, and archaeological discoveries.
A burial site uncovered by archaeologists in southern Siberia has revealed a series of unusual discoveries, which include ritually broken artifacts surrounding human and horse remains.
The burial site was uncovered in the Sayan Mountains and was discovered during a survey in advance of railway construction planned for the area. Archaeologists believe that the grave dates to the tenth century.
Originally uncovered in 2014, excavations at the site of the unusual grave and several others found nearby have been ongoing, revealing discoveries that researchers say are unusual for the period and location.
“It is quite clear that this is far from an ordinary burial,” according to Andrey Poliakov, a researcher with the Russian Academy of Sciences and one of the co-authors of a new study detailing the findings, which appeared in Bulletin of Novosibirsk State University, Series: History, Philology.
Among the unusual discoveries in the Siberian grave were human remains—those belonging to a woman, as well as a newborn child—and animal remains belonging to a horse, including the creature’s skull, skin, and limbs, and a spine that belonged to a sheep.
The woman’s remains were also accompanied by several artifacts, which included bronze earrings, a spindle whorl crafted from stone, and an iron knife. Also associated with the woman’s remains was a mirror, which archaeologists believe may have been intentionally broken for ritual purposes—a practice maintained by many ancient cultures throughout the world.
Also of interest was the design of the mirror, which features carvings of a vine that winds along the artifact and clusters of grapes. The research team believes this could indicate that this artifact, and possibly some of the others found in the grave, may have originated from China.
Additionally, the researchers report that items associated with the remains of the horse contained within the burial site are associated with older periods, which would have made them antiques at the time they were included within the grave. Another example of artifacts with possible eastern origins includes a stirrup, which features decorations that bear resemblance to ancient art found in China and India.
Such stirrups were often employed by nomads who once dwelt on the Eurasian Steppe, and featured on the left side of their saddles—a position which archaeologists also believe may have held ritual significance.
According to the researchers in their study, “The entire set of items constitutes a complete set of riding horse equipment,” which they say has “an archaic appearance and could be preserved for a long time as ‘family heirlooms’.”
The researchers add that such items were particularly popular in ancient nomadic society along the Eurasian Steppe and were viewed “as an indicator of social prestige” during the 8th and 9th centuries.
The unusual collection of artifacts within the grave was considered a rare find for this region, although further interpretation about the remains of the woman and the infant, which may had been her child, may be required.
The paper, “Riding Horse Equipment from a Medieval Burial on the Idzhim River in the Usinskaya Basin,” by Andrey Poliakov and colleagues, appeared in Bulletin of Novosibirsk State University, Series: History, Philology.
Micah Hanks is the Editor-in-Chief and Co-Founder of The Debrief. A longtime reporter on science, defense, and technology with a focus on space and astronomy, he can be reached at micah@thedebrief.org. Follow him on X @MicahHanks, and at micahhanks.com.
Archaeologists say bones unearthed from an Iron Age burial in Scotland are revealing new insights into how human remains were interred in ancient Europe, in a region with scant evidence for mortuary practices in the archeological record.
Among the new findings, cuts within the skull of a woman’s 2,000-year-old Iron Age remains suggest the removal of her brain prior to burial, along with other evidence of mortuary processing.
“Manipulation of one skeleton, including the possible removal of the brain, fashioning of long bones into ‘tools’ and reassembly for burial, suggests complex mortuary processing,” write the authors of a new study published in the journal Antiquity.
The study’s authors report that the woman’s remains, and those believed to have belonged to a teenage boy buried nearby, both had distant origins, adding to the intrigue. Archaeologists discovered the pair buried in a cairn in Sutherland’s Loch Borralie in northwest Scotland, a rare find in such well-preserved condition.
Due to Britain’s acidic peat soil, few Iron Age human remains have been recovered, leaving many questions about the inhabitants’ funerary practices. This particular corner of Scotland is an exception, with an environment better suited to preserving bone, offering a rare window into this mysterious period.
“To find bodies of this age so well preserved is rare, but to discover evidence of a funeral ritual we previously knew nothing about was such a surprise and very exciting,” said lead author Dr. Laura Castells Navarro.
“The adult female displayed incisions on the inside of her cranium, suggesting her brain had been intentionally removed after death,” Castells Navarro continued. “We have no other comparison of this type of practice, but we know that the dead were curated and treated carefully in Iron Age Britain, so our understanding is that this bone modification is within that same tradition of care.”
The sharpened bones, which the archeologists suspected were used as tools, included humeri, ulna, and femur. Why Iron Age Britons would have engaged in such elaborate and unusual manipulation of skeletal remains eludes archaeologists, although they note that the care evident in reassembling her skeleton for burial suggests she commanded the respect and reverence of those who laid her to rest.
Analysis techniques used on the bones include isotope testing and DNA mapping, revealing a close kinship suspected to be that of maternal second cousins.
More intriguing than their relationship is the evidence of the distance they had traveled, as isotopic analysis revealed that the pair had grown up 50 miles to the southeast. Genetics pushed this even further, revealing family connections beyond the mainland, spreading out in opposite directions. To the northeast, they had relatives 108 miles away on the Orkney Islands, while to the southwest, they had kin 140 miles away at Applecross.
Archaeologists interpret this to mean that Iron Age Britons were extremely mobile, traversing water in family groups.
“Our research shows that prehistoric maritime communities periodically moved around the north coast and Northern Isles of Scotland, possibly in small groups,” Dr. Castells Navarro said. “This movement allowed for the spread and maintenance of cultural practices and traditions.”
With individuals of shared kinship spread so far, researchers suggest these complex mortuary practices were likely shared across Iron Age Britain, adding an intriguing new wrinkle to our assessment of these ancient people.
“I think we can assume that the Iron Age dead were not forgotten,” Dr. Castells Navarro concluded, “and still had meaning to the living, with a continued interaction between the living and the dead across time and distance.”
The paper, “Reconnecting the Dead in Iron Age Britain: Funerary Processing and Long-Distance Connectivity at Loch Borralie, Scotland,” appeared in Antiquity on June 10, 2026.
Ryan Whalen covers science and technology for The Debrief. He holds an MA in History and a Master of Library and Information Science with a certificate in Data Science. He can be contacted at ryan@thedebrief.org, and follow him on Twitter @mdntwvlf.
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