A team of researchers examined 23 teeth from at least 20 individuals from South Africa‘s Rising Star Cave system and found no evidence of the male-specific Amelogenin-Y protein.

A recent study, published in Cell, marks the first ancient protein analysis of Homo naledi and raises the possibility that the Dinaledi Chamber may contain predominantly, or possibly exclusively, female individuals. If confirmed, Homo naledi would become the first known non-human species to practice sex-specific burial.

A Decade-Old Puzzle

Since its discovery in 2013, Homo naledi has puzzled paleoanthropologists. This extinct human relative lived between 335,000 and 241,000 years ago and combined ape-like anatomy with distinct human features. So far, Homo naledi remains have only been found in the Rising Star Cave system in South Africa.

For over a decade, scientists noted that the adult fossils from the Dinaledi Chamber were nearly identical, lacking the skeletal variation usually seen between males and females. The reason for this similarity remained unclear.

Proteins Where DNA Fails

To investigate further, a team from the University of York, the University of Copenhagen, and additional institutions analyzed the remains at the molecular level. They used a minimally destructive acid-etching method to extract protein fragments, or peptides, from the enamel of the 23 teeth.

“Unlike those found in other remains like bone fragments, proteins in tooth enamel are preserved because dental enamel—the hardest tissue in the human body—shields proteins from environmental contamination even for millions of years. This makes them ideal carriers of genetic information from deep time. Our study helps explain the long-standing mystery of why Homo naledi lacked significant variation; it’s probably because they could have all belonged to one sex,” said Palesa Madupe, who conducted the research during her postdoctoral fellowship at the University of Copenhagen.

A Missing Male Marker

The researchers specifically searched for Amelogenin-Y, a protein produced only by the male Y chromosome, but detected none of it in the sampled teeth. To make sure the results were not due to modern contamination, a specialized chemistry team at the University of York independently confirmed that the amino acids were genuinely ancient. The findings remained consistent.

“The lack of male markers within the group is truly fascinating. It is incredibly exciting to gain a window not only into the biology of our ancestors, but also into how they lived. These findings offer rare insights into a culture that has, until now, been difficult to access directly. Advances in ancient protein analysis are opening the door to a far richer and more nuanced understanding of ancient hominins,” said Dr. Marc Dickinson from the university’s Department of Chemistry.

Ritual or Genetic Fluke?

The findings allow for two possible explanations. One is cultural: if the chamber were reserved for females, it could indicate that Homo naledi practiced sex-specific, symbolic mortuary behavior, a trait previously thought to be unique to modern humans.

The second explanation is biological. The researchers note that a small, isolated Homo naledi population could have lost or mutated the Amelogenin-Y gene, leaving males without the protein marker typically used to identify male remains.

Either explanation could reshape what scientists know about Homo naledi. If researchers confirm the site as an all-female burial chamber, it would suggest that symbolic behavior emerged earlier in the human lineage than previously believed. On the other hand, a genetic change across the population would show how isolation can shape a species’ biology in ways that fossils alone cannot reveal. This study shows how ancient protein analysis from teeth can open new windows into human evolution.

Austin Burgess is a writer and researcher with a background in sales, marketing, and data analytics. He holds an MBA, a Bachelor of Science in Business Administration, and a data analytics certification. His work focuses on breaking scientific developments, with an emphasis on emerging biology, cognitive neuroscience, and archaeological discoveries.

In the literature related to the powerful entheogen dimethyltriptamine (DMT), it is often reported that individuals claim to have had encounters with what they perceived as non-human entities during their DMT experiences. Descriptions related to these powerful visionary experiences range from what some describe as insect-like forms and machine-like intelligences, to what some describe as conscious entities associated with intricate geometries.

For years, many researchers have regarded these reports purely as hallucinations generated by the human brain while under the influence of the psychedelic compound. However, past studies have never directly tested whether these reported entities could represent something other than internally generated hallucinations.

Now, a recent preprint paper outlines a path toward testing this assumption experimentally.

A collaborative team from the Trace Institute and the nonprofit Noonautics has released a preprint titled Traces of the Other –Are DMT Entities Real? DMT Phenomenology in the Framework of Conscious Realism. The study presents a mathematical framework for understanding the human psychedelic experience and outlines experiments designed to test its primary hypothesis.

The research was led by Professor Emeritus Donald Hoffman, founder of the Trace Institute, and Dr. Andrew Gallimore, a neurobiologist and pharmacologist at the Okinawa Institute of Science and Technology and head of Noonautics. The preprint is currently awaiting peer review.

A Problem With a Default Answer

According to some figures, as many as 45% of the individuals who take a sufficient dose of DMT report experiences involving non-human beings. People describe these entities as intelligent and autonomous, and report similar experiences across different cultures and backgrounds.

Typical hallucinations tend to draw on material from memory, such as familiar faces, animals, or places. By contrast, encounters with other humans appear in less than 5% of DMT encounters.

The authors of the new preprint study argue that these reports are difficult to reconcile with conventional accounts of pure hallucination and that the consistency of reports regardless of culture, location, belief systems, and other factors warrants deeper investigation. 

A Framework Built on Conscious Realism

The project draws on Hoffman’s theory of conscious realism, which proposes that interacting conscious agents fundamentally constitute reality. In this framework, the physical world as humans perceive it functions more like a user interface than a direct view of objective reality. According to this theory, conscious agents of varying levels of complexity may exist beyond what humans normally perceive and go unnoticed in everyday life.

The researchers propose that DMT changes the perceptual interface, allowing consciousness to access regions where normal perceptual constraints are absent. In these regions, it may be possible to detect traces of other conscious agents. Hoffman says the project “will provide a new framework for exploring the effects of psychoactive substances such as DMT on the structure and function of spacetime.”

The Protocol That Makes Testing Possible

Testing these ideas has become possible due to past work involving technical development by Gallimore and researcher Rick Strassman. Typically, a DMT experience lasts only three to five minutes, which is not long enough for structured experiments. The extended-state DMT protocol, called DMTx, uses a controlled intravenous infusion to maintain a stable experience for up to an hour, allowing researchers to adjust the dosage as required.

That controllable window makes experimentation possible. “With a theoretical foundation for the highly unusual state of consciousness induced by DMT, we can test these theories experimentally,” Gallimore says.

Experiments Built to Fail

The proposed experiments are designed so that if the external-agent hypothesis is incorrect, the results will be negative. In one protocol, a computer in a sealed room randomly displays either blue or yellow, and a subject under DMTx attempts to learn the hidden color by interacting with an entity. Another experiment involves two isolated subjects under DMTx who try to transmit a random word to each other through a shared entity, a process that would be difficult to explain solely in terms of internal brain mechanisms.

The researchers acknowledge several limitations. Cooperation from entities cannot be guaranteed, results depend on subjective reports, and expectation bias is difficult to rule out. The preprint continues to treat the hallucination model as the default explanation for these entities. What distinguishes this work is that the hypothesis is now formulated in a way that allows for experimental testing.

“This collaboration is a first step to a mathematics of altered states of consciousness and, ultimately, for engineering our perceptual interface to expand our view of reality,” Gallimore says.

Hoffman and Gallimore will discuss the research publicly on Saturday, June 13, 2026, at the Lighthouse Campus at the historic Venice Beach Post Office in Los Angeles, with video to follow on YouTube.

Austin Burgess is a writer and researcher with a background in sales, marketing, and data analytics. He holds an MBA, a Bachelor of Science in Business Administration, and a data analytics certification. His work focuses on breaking scientific developments, with an emphasis on emerging biology, cognitive neuroscience, and archaeological discoveries.