A team of researchers examined 23 teeth from at least 20 individuals from South Africa‘s Rising Star Cave system and found no evidence of the male-specific Amelogenin-Y protein.

A recent study, published in Cell, marks the first ancient protein analysis of Homo naledi and raises the possibility that the Dinaledi Chamber may contain predominantly, or possibly exclusively, female individuals. If confirmed, Homo naledi would become the first known non-human species to practice sex-specific burial.

A Decade-Old Puzzle

Since its discovery in 2013, Homo naledi has puzzled paleoanthropologists. This extinct human relative lived between 335,000 and 241,000 years ago and combined ape-like anatomy with distinct human features. So far, Homo naledi remains have only been found in the Rising Star Cave system in South Africa.

For over a decade, scientists noted that the adult fossils from the Dinaledi Chamber were nearly identical, lacking the skeletal variation usually seen between males and females. The reason for this similarity remained unclear.

Proteins Where DNA Fails

To investigate further, a team from the University of York, the University of Copenhagen, and additional institutions analyzed the remains at the molecular level. They used a minimally destructive acid-etching method to extract protein fragments, or peptides, from the enamel of the 23 teeth.

“Unlike those found in other remains like bone fragments, proteins in tooth enamel are preserved because dental enamel—the hardest tissue in the human body—shields proteins from environmental contamination even for millions of years. This makes them ideal carriers of genetic information from deep time. Our study helps explain the long-standing mystery of why Homo naledi lacked significant variation; it’s probably because they could have all belonged to one sex,” said Palesa Madupe, who conducted the research during her postdoctoral fellowship at the University of Copenhagen.

A Missing Male Marker

The researchers specifically searched for Amelogenin-Y, a protein produced only by the male Y chromosome, but detected none of it in the sampled teeth. To make sure the results were not due to modern contamination, a specialized chemistry team at the University of York independently confirmed that the amino acids were genuinely ancient. The findings remained consistent.

“The lack of male markers within the group is truly fascinating. It is incredibly exciting to gain a window not only into the biology of our ancestors, but also into how they lived. These findings offer rare insights into a culture that has, until now, been difficult to access directly. Advances in ancient protein analysis are opening the door to a far richer and more nuanced understanding of ancient hominins,” said Dr. Marc Dickinson from the university’s Department of Chemistry.

Ritual or Genetic Fluke?

The findings allow for two possible explanations. One is cultural: if the chamber were reserved for females, it could indicate that Homo naledi practiced sex-specific, symbolic mortuary behavior, a trait previously thought to be unique to modern humans.

The second explanation is biological. The researchers note that a small, isolated Homo naledi population could have lost or mutated the Amelogenin-Y gene, leaving males without the protein marker typically used to identify male remains.

Either explanation could reshape what scientists know about Homo naledi. If researchers confirm the site as an all-female burial chamber, it would suggest that symbolic behavior emerged earlier in the human lineage than previously believed. On the other hand, a genetic change across the population would show how isolation can shape a species’ biology in ways that fossils alone cannot reveal. This study shows how ancient protein analysis from teeth can open new windows into human evolution.

Austin Burgess is a writer and researcher with a background in sales, marketing, and data analytics. He holds an MBA, a Bachelor of Science in Business Administration, and a data analytics certification. His work focuses on breaking scientific developments, with an emphasis on emerging biology, cognitive neuroscience, and archaeological discoveries.

A burial site uncovered by archaeologists in southern Siberia has revealed a series of unusual discoveries, which include ritually broken artifacts surrounding human and horse remains.

The burial site was uncovered in the Sayan Mountains and was discovered during a survey in advance of railway construction planned for the area. Archaeologists believe that the grave dates to the tenth century.

Originally uncovered in 2014, excavations at the site of the unusual grave and several others found nearby have been ongoing, revealing discoveries that researchers say are unusual for the period and location.

“It is quite clear that this is far from an ordinary burial,” according to Andrey Poliakov, a researcher with the Russian Academy of Sciences and one of the co-authors of a new study detailing the findings, which appeared in Bulletin of Novosibirsk State University, Series: History, Philology.

Curious Discoveries in a Siberian Grave

Among the unusual discoveries in the Siberian grave were human remains—those belonging to a woman, as well as a newborn child—and animal remains belonging to a horse, including the creature’s skull, skin, and limbs, and a spine that belonged to a sheep.

The woman’s remains were also accompanied by several artifacts, which included bronze earrings, a spindle whorl crafted from stone, and an iron knife. Also associated with the woman’s remains was a mirror, which archaeologists believe may have been intentionally broken for ritual purposes—a practice maintained by many ancient cultures throughout the world.

Eastern Connections

Also of interest was the design of the mirror, which features carvings of a vine that winds along the artifact and clusters of grapes. The research team believes this could indicate that this artifact, and possibly some of the others found in the grave, may have originated from China.

Additionally, the researchers report that items associated with the remains of the horse contained within the burial site are associated with older periods, which would have made them antiques at the time they were included within the grave. Another example of artifacts with possible eastern origins includes a stirrup, which features decorations that bear resemblance to ancient art found in China and India.

Such stirrups were often employed by nomads who once dwelt on the Eurasian Steppe, and featured on the left side of their saddles—a position which archaeologists also believe may have held ritual significance.

An Elite Burial from the 10th Century

According to the researchers in their study, “The entire set of items constitutes a complete set of riding horse equipment,” which they say has “an archaic appearance and could be preserved for a long time as ‘family heirlooms’.”

The researchers add that such items were particularly popular in ancient nomadic society along the Eurasian Steppe and were viewed “as an indicator of social prestige” during the 8th and 9th centuries.

The unusual collection of artifacts within the grave was considered a rare find for this region, although further interpretation about the remains of the woman and the infant, which may had been her child, may be required.

The paper, “Riding Horse Equipment from a Medieval Burial on the Idzhim River in the Usinskaya Basin,” by Andrey Poliakov and colleagues, appeared in Bulletin of Novosibirsk State University, Series: History, Philology.

Micah Hanks is the Editor-in-Chief and Co-Founder of The Debrief. A longtime reporter on science, defense, and technology with a focus on space and astronomy, he can be reached at micah@thedebrief.org. Follow him on X @MicahHanks, and at micahhanks.com.

Archaeologists say bones unearthed from an Iron Age burial in Scotland are revealing new insights into how human remains were interred in ancient Europe, in a region with scant evidence for mortuary practices in the archeological record.

Among the new findings, cuts within the skull of a woman’s 2,000-year-old Iron Age remains suggest the removal of her brain prior to burial, along with other evidence of mortuary processing. 

“Manipulation of one skeleton, including the possible removal of the brain, fashioning of long bones into ‘tools’ and reassembly for burial, suggests complex mortuary processing,” write the authors of a new study published in the journal Antiquity. 

Iron Age Remains

The study’s authors report that the woman’s remains, and those believed to have belonged to a teenage boy buried nearby, both had distant origins, adding to the intrigue. Archaeologists discovered the pair buried in a cairn in Sutherland’s Loch Borralie in northwest Scotland, a rare find in such well-preserved condition. 

Due to Britain’s acidic peat soil, few Iron Age human remains have been recovered, leaving many questions about the inhabitants’ funerary practices. This particular corner of Scotland is an exception, with an environment better suited to preserving bone, offering a rare window into this mysterious period.

“To find bodies of this age so well preserved is rare, but to discover evidence of a funeral ritual we previously knew nothing about was such a surprise and very exciting,” said lead author Dr. Laura Castells Navarro.

Unusual Mortuary Practices

“The adult female displayed incisions on the inside of her cranium, suggesting her brain had been intentionally removed after death,” Castells Navarro continued. “We have no other comparison of this type of practice, but we know that the dead were curated and treated carefully in Iron Age Britain, so our understanding is that this bone modification is within that same tradition of care.”

The sharpened bones, which the archeologists suspected were used as tools, included humeri, ulna, and femur. Why Iron Age Britons would have engaged in such elaborate and unusual manipulation of skeletal remains eludes archaeologists, although they note that the care evident in reassembling her skeleton for burial suggests she commanded the respect and reverence of those who laid her to rest.

Analysis techniques used on the bones include isotope testing and DNA mapping, revealing a close kinship suspected to be that of maternal second cousins.

Iron Age Connections

More intriguing than their relationship is the evidence of the distance they had traveled, as isotopic analysis revealed that the pair had grown up 50 miles to the southeast. Genetics pushed this even further, revealing family connections beyond the mainland, spreading out in opposite directions. To the northeast, they had relatives 108 miles away on the Orkney Islands, while to the southwest, they had kin 140 miles away at Applecross.

Archaeologists interpret this to mean that Iron Age Britons were extremely mobile, traversing water in family groups.

“Our research shows that prehistoric maritime communities periodically moved around the north coast and Northern Isles of Scotland, possibly in small groups,” Dr. Castells Navarro said. “This movement allowed for the spread and maintenance of cultural practices and traditions.”

With individuals of shared kinship spread so far, researchers suggest these complex mortuary practices were likely shared across Iron Age Britain, adding an intriguing new wrinkle to our assessment of these ancient people.

“I think we can assume that the Iron Age dead were not forgotten,” Dr. Castells Navarro concluded, “and still had meaning to the living, with a continued interaction between the living and the dead across time and distance.”

The paper, “Reconnecting the Dead in Iron Age Britain: Funerary Processing and Long-Distance Connectivity at Loch Borralie, Scotland,” appeared in Antiquity on June 10, 2026.

Ryan Whalen covers science and technology for The Debrief. He holds an MA in History and a Master of Library and Information Science with a certificate in Data Science. He can be contacted at ryan@thedebrief.org, and follow him on Twitter @mdntwvlf.